Journal: Journal of Biomedical Optics

Article Title: Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuse in vivo flow cytometry

doi: 10.1117/1.JBO.31.2.027001

Figure Lengend Snippet: Flow cytometry analysis of targeted contrast agent-labeled cancer cells and peripheral blood mononuclear cells (PBMCs). Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.

Article Snippet: Peripheral blood mononuclear cells (PBMCs; PCS-800-011; ATCC) were thawed and prepared according to ATCC instructions for in vitro non-specific contrast agent uptake studies.

Techniques: Flow Cytometry, Labeling, Incubation, Expressing

Journal: Journal of Biomedical Optics

Article Title: Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuse in vivo flow cytometry

doi: 10.1117/1.JBO.31.2.027001

Figure Lengend Snippet: Flow cytometry analysis of targeted contrast agent-labeled cancer cells and PBMCs. Suspensions of 1:1000 LNCaP prostate cancer cells to PBMCs were incubated (a) without and (b) with PSMA-02. (c) Different PSMA-expressing cancer cell lines and PBMCs incubated with PSMA-02 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and Red-NIR-DiFC detection threshold (green line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LNCaP prostate cancer to PBMCs were incubated (d) without and (e) with PSMA-04. (f) Different PSMA-expressing cancer cell lines and PBMCs incubated with PSMA-04 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and Red-NIR-DiFC detection threshold (green line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.

Article Snippet: Peripheral blood mononuclear cells (PBMCs; PCS-800-011; ATCC) were thawed and prepared according to ATCC instructions for in vitro non-specific contrast agent uptake studies.

Techniques: Flow Cytometry, Labeling, Incubation, Expressing

Journal: bioRxiv

Article Title: UVB-Induced Genotoxic Stress Activates the DNA Damage Response and Innate Immune Pathways in Sea Urchin Coelomocytes

doi: 10.64898/2026.01.14.699502

Figure Lengend Snippet: (a) Viability of S. purpuratus coelomocytes and human peripheral blood mononuclear cells (PBMCs) measured 24 h after exposure to a range of UVB doses. For coelomocytes, mean values ± the standard deviation of biological triplicates (n = 3) are shown. For PBMCs, mean values ± the standard deviation of technical triplicates are shown. Individual points are overlaid as white circles. (b) Example of a comet with the head and tail region indicated. (c) Average tail DNA percent and (d) average percent of damaged cells (the percent of cells with any amount of DNA damage) in S. purpuratus coelomocytes challenged with 1,000 mJ/cm 2 UVB at 1, 6, and 24 h post-exposure (red bars) versus control, untreated coelomocytes (purple bars). Error bars are mean values ± the standard deviation of biological triplicates (n = 3). Individual points are overlaid as white circles. Significant differences between control and UVB-treated cells were analyzed using a one-way ANOVA and post-hoc TukeyHSD test (***, adjusted p value < 1 x 10 -3 ).

Article Snippet: In contrast, human peripheral blood mononuclear cells (PBMCs; ATCC PCS-800-011) demonstrated a sharp decline in viability following exposure to UVB with an LD 50 value of 871 mJ/cm 2 .

Techniques: Standard Deviation, Control